Composite
Part:BBa_K190037
Designed by: Michael Verhoeven Group: iGEM09_Groningen (2009-09-07)
GVP with pBad/araC promoter
The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the pBad/araC promoter BBa_K113009 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1552
Illegal BamHI site found at 1144
Illegal BamHI site found at 5357
Illegal XhoI site found at 5045 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2003
Illegal NgoMIV site found at 2405
Illegal AgeI site found at 979
Illegal AgeI site found at 5324 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 3815
Illegal SapI.rc site found at 4971
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Categories
Parameters
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