Composite

Part:BBa_K190037

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)


GVP with pBad/araC promoter

The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the pBad/araC promoter BBa_K113009 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1552
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 5357
    Illegal XhoI site found at 5045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2003
    Illegal NgoMIV site found at 2405
    Illegal AgeI site found at 979
    Illegal AgeI site found at 5324
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 3815
    Illegal SapI.rc site found at 4971


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Categories
Parameters
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